Global Conference on Nanomedicine, Nanobiology, Nanotechnology & Pharmacology
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Accepted Abstracts

Novel approach of next generation sequencing in forensic genetics

Muhammad Umair*¹, Faryal Batool²
1.University of Punjab, Pakistan
2. Bahauddin Zakariya University, Pakistan

Citation: Umair M, Batool F (2019) Novel approach of next generation sequencing in forensic genetics. SciTech Nanosciences-Pharma 2019.Tokyo: Japan

Received: May 10, 2019         Accepted: May 13, 2019         Published: May 13, 2019

Abstract

Next Generation Sequencing is advanced method which can perform sequencing of millions of copies of genes in parallel manner. Bioinformatics tools are widely used in NGS. Sequencing of genome of different organisms started ten years ago and now it is possible for us to find the complete genomic sequence of most of organisms. Now we can use this data for clinical diagnosis of different diseases. More information may be obtained from unique samples in a single experiment by analyzing combinations of markers. ThermoFisher Scientific launched two SNP typing assays in 2014 designed for the Ion PGM System. NGS makes it possible to expand forensic genetic investigations to new areas related to forensic medicine. Previously unknown STR alleles have been discovered Sequencing was conducted with the Sanger dideoxynucleotide (ddNTP) chain terminating method, where the incorporation of ddNTP to a growing DNA chain prevented further extension by the DNA polymerase. Early on, the synthesized DNA fragments were separated by slab gel electrophoresis and detected either radioactively or fluorescently labeled deoxynucleotides (dNTPs) incorporated into the DNA fragments. Subsequent introduction of fluorescently labeled ddNTPs and capillary electrophoresis (CE) platforms increased sensitivity and decreased the cost of Sanger sequencing to a level where sequencing of complete genomes became possible. The detailed sequence information may aid mixture interpretation and will increase the statistical weight of the evidence When a person dies unexpectedly and for no apparent reason, shotgun or exome sequencing may identify genetic variants associated with known diseases and assist the pathologist in finding the cause of death. The advantages of NGS compared to the traditional PCR-CE methods are many, and there is little doubt that NGS will be implemented and used in forensic laboratories in the future. Prices of instruments and kits will determine how fast the transition from CE to NGS will be and how large a fraction of cases will be investigated by NGS.