This paper is the first attempt to exhibit anti-bacterial activity of copper oxide nanoparticles synthesized using traditionally prepared dry powder and extract of Terminalia chebula through a simple and efficient microwave combustion method. As it is believed that copper is the essential mineral for humans to kill pathogens and T.chebula boosts immunity, this method of derived nano copper oxide is considered as an efficient biomedical applicant. The results from X-ray diffraction showed the crystalline formation of monoclinic structured copper oxide nanoparticleswhose crystalline size was found to be 18 nm and 15 nm, scanning electron microscopereveals spherical, clustered and surface rough aggregated copper oxide nanoparticles with varying sizes ranged between 12-18 nm which is evidenced from X-ray diffraction results. Energy dispersive spectroscopyrevealed presence of 19.95 wt% of O and75.66 wt% of Cu, lesser amount of K, Cl, Ca (3.63 wt%) present in the spectra may be owing to the presence of phyto-chemicals constituent in the fruit. The energy dispersive spectrum clearly reveals the material is free from impurities other than fruit constituent.The strong band formation at 531 and 538 cm^-1in fourier transform infrared spectroscopy confirms metal oxide formation. The higher band gap energyof 5.3 eV was found from ultraviolet-visible spectroscopy esteemed using tauc plot. Thermogravimetric/Diffraction thermal analyser curve confirms the material is stable at high temperature. The results fromdielectric studiesshows decrease in dielectric constant and dielectric loss with increase in frequency. This makes the sample a significant parameter for enhancing antimicrobial activity. The antimicrobial activity of copper oxide nanoparticles obtained from extract showed more zone of inhibition recorded against S.aureusand S.typhiand less zone of inhibition for C.albicans thancopper oxide nanoparticles derived from dry powder of T.chebula. These results justify that T.chebuladerived copper oxide nanoparticles can effectively be used for therapeutic purposes.

Keywords: CuO Nanoparticles, T.chebula, Microwave synthesis, Antimicrobial activity