The damage of the liver is often accompanied by a disruption of the bile excretory functions of the liver. The ability of the hepatoprotectors to positively influence on the cholestasis state is an important pharmacological characteristic. Phytomedicines from Tanacetum vulgare flowers are lacking in the pharmaceutical market of Ukraine.
The aim – Evaluation of Tanacetum vulgare flowers extract (TVFE) efficacy in a model of toxic hepatitis in rats. Model hepatitis (MH) was reproduced by a subcutaneous administration of tetrachloromethane 50% oil solution in a dose of 0.4 ml/100 g of body weight with subsequent intragastric 40% ethyl alcohol introduction (1.3 ml/100 g of body weight) for 4 days. Male rats weighing 190–220 g were divided into eight groups: intact control; MH; 5 groups - animals with MH, which were administered TVFE in doses of 25, 50, 75, 100, and 150 mg/kg, respectively; animals with MH, which were treated with reference medicine (RM) silymarin (Karsil, Sopharma, Bulgaria) at a dose of 100 mg/kg. TVFE was administered 1 time/day 7 days before and during MH. On the 4th day of MH the influence of TVFE on the bile formation and secretion, state of cytolysis and antioxidant-prooxidative processes were studied. It has been established that TVFE has dose-dependent hepatoprotective and choleretic effects: in doses of 75 and 100 mg/kg TVFE decreased the level of thiobarbituric acid active products, increased the concentration of reduced glutathione, decreased the activity of alanine aminotransferase. TVFE only in a dose of 75 mg/kg significantly increased the bile acids and cholesterol content, influenced the total volume of bile, and decreased alkaline phosphatase activity. TVFE in a dose of 75 mg/kg was found to be most effective.
Key words: Tanacetum vulgare flowers extract, hepatoprotective effect, choleretic effect, toxic hepatitis in rats.