Global Conference on Nanomedicine, Nanobiology, Nanotechnology & Pharmacology
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Accepted Abstracts

Phytochemical Analysis, Antimicrobial and antioxidant Activity of Essential Oil from Hibiscus sabdariffa (L) Seeds, (Sudanese Karkadi).

Tuhami Elzein Hagr*1, Ibrahim Abdurrahman Adam2
University of Bahri, Sudan

University of Zalingei, Sudan

Citation: Hagr TE, Adam IA (2019) Phytochemical Analysis, Antimicrobial and antioxidant Activity of Essential Oil from Hibiscus sabdariffa (L) Seeds, (Sudanese Karkadi). SciTech Nanosciences-Cancer Sciences 2019. Tokyo: Japan

Received: August 21, 2019         Accepted: August 22, 2019         Published: August 22, 2019

Abstract

The aims of the present study are to assess the phytochemical screening, investigate the chemical constituents of the Essential Oil from H. sabdariffa (L) Seeds, (Sudanese Karkadi) and to evaluate its potential antibacterial and antioxidant activities. Using Soxhlet method to extract the essential oil from H. sabdariffa (L) Seeds. The chemical constituents of H. sabdariffa (L) Oil were identified and quantified by GC-MS, where DPPH and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. Phytochemical screening results showed that Alkaloids, Flavonoid, Carbohydrate, Saponins, Triterpen, Streol, Tannins and phenolic compounds are present in seeds of the H. sabdariffa. Thirty eight components has been identified which classified in to four categories; 31 fatty acid ester derivatives , the majority of them are: Hexadecanoic  acid, methyl ester (16.94%),9,12- Octadecadienoic acid(Z,Z) -, methyl ester (21.93%) ,9, -Octadecadienoic acid (Z)-, methyl ester (30.11%), methyl stearate(7.39%), Cyclopropaneoctanoic   acid (3.17 %)  , Dotriacontane(2.17 %), three phenolic derivatives, 1,3-Benzodioxole,4methoxy-6-(2-propenyl)- (0.01  %)  , Apiol (0.04  %) and  17 Androstannone, 3-(3, 4-dimethylphenyl) (1.81  %)   , three Pentacyclic triterpenes derivatives, Beta.-Amyrin (3.82%) (3.17 %), Alpha.-Amyrin (1.65  %)   and Dodecanoic acid ,methyl ester (0.01 %), and one stroide derivative, Stigmasta-4,7,22-trien-3.beta.-ol (0.41%).The DPPH assay, showed moderate antioxidant potential (50 ± 0.01 compared with standard 89 ± 0.01; the antibacterial showed high inhibitory effect against Bacillus subtilis (13mm). In conclusion, the results showed that the Oil of H. sabdariffa seeds is a good source of antioxidants due to the presence of phenolic compounds, also is a potential source of natural antibacterial, and   justify its uses in folkloric medicines.
Keywords:   Phytochemical Screening, GC-MS Analysis, Antimicrobial and antioxidant Activity