Plant-derived nanoparticles produced by readily available plant materials and the nontoxic nature of plants are suitable for fulfilling the high demand for nanoparticles with applications in the biomedical and environmental areas. In this present paper, we demonstrated a hitherto green biological route for the synthesis of silver nanoparticles using an extract derived from S. virginianum fruit. Our keen interest is to scrutinize the inhibitory effect of synthesized AgNPs against several pathogenic bacteria and fungi. Inhibitory effect was found to be maximum in 400µl of synthesized Agnp against S. epidermis(24 mm) and Bacillus sp.(23.3 mm), whereas minimum in F. oxysporum(12 mm). Uv- visible spectroscopy profile confirms the presence of peak at 240 nm. Spectral assignments indicate that the wavenumber at 1021.12^cm-1 denoted the stretching vibrations of primary amine, CN stretch. Therefore, utilization of plants for the green synthesis is beneficial than other sources while it is economical and might be scaled up for large-scale production.