Global Congress on Infectious Diseases & HIV/AIDS
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Accepted Abstracts

Antimicrobial Resistance Patterns and Molecular Characterization of Klebsiella pneumoniae in Clinical Isolates at Mbarara Regional Referral Hospital

Joseph Turugurwa1*, James Mwesigye1, Kennedy Kassaza1, Fredrick Byarugaba1, Taseera Kabanda1, Benson Musinguzi2
1 Mbarara University of Science and Technology, Uganda
2 Kampala International University, Uganda

Citation: Turugurwa J, Mwesigye J, Kassaza K, Byarugaba F, Kabanda T et al (2020) Antimicrobial Resistance Patterns and Molecular Characterization of Klebsiella pneumoniae in Clinical Isolates at Mbarara Regional Referral Hospital. SciTech Infectious Diseases 2020. Mauritius 

Received: January 13, 2020         Accepted: January 16, 2020         Published: January 16, 2020

Abstract

Background:  Klebsiella pneumoniae is one of the most frequent opportunistic pathogens causing a range of infections and producing enzymes ESBLs, carbapenemases which are responsible for its resistance to beta-lactamases antibiotics including carbapenems.
Aim: The aim of the present study was to determine the antimicrobial resistance patterns and molecular characterization of Klebsiella pneumoniae in clinical isolates at Mbarara Regional Referral Hospital (MRRH)
Materials and Methods: A laboratory-based descriptive cross-sectional study that was conducted from September 2018 to May 2019 at MRRH. Klebsiella pneumoniae was identified by cultural and biochemical methods. Antibiotic sensitivity test was performed by modified Kirby-Bauer disc diffusion technique. ESBL production in Klebsiella pneumoniae was tested by double disc synergy test, Carbapenemase production by MHT, Boronic Acid or EDTA test using Meropenem phenotypically and both resistance confirmed genotypically by Multiplex PCR.
Results: Out of 1055 clinical isolates, 298 (28.2%) were found positive for Klebsiella.spp, 175 isolates were subcultured among which 22 (12.57%) were K. pneumoniae based on API 20E. Overall Sensitivity patterns of these Klebsiella pneumoniae isolates to Ceftriaxone, (Amoxicillin/Clavulanate), Gentamicin, Cefepime, Ciprofloxacin, Cefoxitin, Nitrofurantoin, Cefuroxime, piperacillin/tazobactam, Meropenem, Ceftazidime and cefotaxime were 72.7%, 63.7%, 54.5% , 45.5%, 31.8%, 31.8%, 27.3%, 27.3%, 22.7%, 22.7%, 18.2%, 9.1%, 9.1%  respectively. ESBL producing K. pneumoniae was found at 68.18% (15/22) phenotypically. Genotypically; the ESBL genes were blaCTX-M (100%), blaSHV (80%) and blaTEM (100; 47%); 8/15 (73.3%) had CTX-M, SHV, TEM, 4/15 (26.67%) CTX-M, TEM, 3/15 (20.00%)  CTX-M and SHV. Carbapenemase producing K. pneumoniae was found at 31.82% (7/22) phenotypically; 1/7 (14.28%) by MHT, 4/7 (57.14%)  Boronic acid test and 2/7 (28.58%) EDTA test. Genotypically; 3/4 [(75%) 42.86%] had OXA-48, 1/4 [(25%) 14.28%] OXA-48 and KPC gene, 1/2 [(50%) 14.28%] KPC and VIM, 1/2 [(50%) 14.28%] KPC and KPC gene [(100%) 14.28%]. Antimicrobial susceptibility patterns of the genotypically confirmed isolates showed that 31.8% were resistant to Ciprofloxacin, 18.2% Meropenem, 13.63% Colistin, 9.09% Ceftazidime/avibactam and Levofloxacin, 4.54% Ertapenem and there was no resistance for Imipenem.
Conclusion/Recommendations: A high prevalence of ESBLs producing Klebsiella pneumoniae (68.18%) and Carbapenem production at 38.82% was observed which calls for routine screening of this resistance phenotypically.
Keywords: Antimicrobial Resistance Patterns, ESBLs, Carbapenemase Resistance, Klebsiella pneumoniae