Received: January 24, 2020 Accepted: January 27, 2020 Published: January 27, 2020
Characterized genotypically ESBL / AmpC / OXA-48 genes in clinical and environmental enterobacteria.The strains were identified by Api 20E and confirmed MALDI-TOF-MS. Antibiotic sensitivity achieved by diffusion on MH agar, as well as the ESBL phenotype. Antibiotic resistance genes were characterized by PCR and sequencing. Sequences were assembled by Codon Code Aligner and compared by BlastX with Genbank, NCBI and ARGANNOT. The phylogenic trees were constructed by MEGA7. Gene transfer resistance achieved by conjugation using E. coli J53 as the recipient strain. The clonal relationship between the strains was made by MLST. 209 strains were isolated: 56 environmental and 153 clinics. 95 strains (45.45%) were ESBL. E. coli, K. pneumoniae, 7 Ent. cloacae, and 3 C. freundii. 3 P. mirabilis, 3 S. marcescens and 1 Arizona spp. Except for imipenem, a total resistance to the majority of beta-lactams, as well as Aminoglycosides and Fluoroquinolones. TEM-1 were predominant (42.10%), followed by CTX-M-15 (32.63%). ACT-1 was found in 6 strains and CMY-2 in 2 strains; OXA-48 in 5 strains. MLST showed that the two strains K. pneumoniae OXA-48 were genetically different ST15 and ST460. Conjugation and transformation showed that blaOXA-48 was not carried by a plasmid. Our study revealed the diffusion of ESBL-type ESBL, CTX-M15 and AmpC in our hospitals that exposes to a growing problem of therapeutic management.