Global Congress on Infectious Diseases & HIV/AIDS
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Accepted Abstracts

New Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss

Janaína C Rodrigues 1,2*, André L L Bachi 3,4,5, Gleiciele A V Silva 2, Marcelo Rossi 3, Jonatas B do Amaral 3, Karina Lezirovitz 1,2 and Rubens de Brito 1
 
1 University of São Paulo (USP), Brazil
2 Laboratory of Otolaryngology (LIM32), School of Medicine, University of São Paulo (USP), Brazil
3 Federal University of São Paulo (UNIFESP), Brazil
4 Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), Brazil
5 Santo Amaro University (UNISA),  Brazil

Citation: Rodrigues JC, Bachi ALL, Silva GAV, Rossi M, Amaral JB et al (2020) New Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss. SciTech Infectious Diseases 2020. Mauritius 

Received: April 14, 2020         Accepted: April 16, 2020         Published: April 16, 2020

Abstract

Noise exposure represents the second most common cause of acquired sensorineural hearing loss and we observed that tumor necrosis factor α (TNFα) was involved in this context. The effect of Tnfα gene silencing on the expression profile related to the TNFα metabolic pathway in an experimental model of noise-induced hearing loss had not previously been studied. Methods: Single ears of Wistar rats were pretreated with Tnfα small interfering RNA (siRNA) by trans-tympanic administration 24 h before they were exposed to white noise (120 dBSPL for three hours). After 24 h of noise exposure, we analyzed the electrophysiological threshold and the amplitude of waves I, II, III, and IV in the auditory brain response click. In addition, qRT-PCR was performed to evaluate the TNFα metabolic pathway in the ears submitted or not to gene silencing. Results: Preservation of the electrophysiological threshold and the amplitude of waves was observed in the ears submitted to gene silencing compared to the ears not treated. Increased anti-apoptotic gene expression and decreased pro-apoptotic gene expression were found in the treated ears. Conclusion: Our results allow us to suggest that the blockade of TNFα by gene silencing was useful to prevent noise-induced hearing loss.
 
Keywords: TNFα metabolic pathway, Apoptosis, Auditory brain response, Cochlea, Electrophysiological threshold, In vivo siRNA administration, Synaptopathy