Background: The ever-increasing multi drug resistant strains of pathogenic bacteria isolated from wound infections, makes it difficult in treating them for clinicians. To evaluate, and hence to elucidate proper treatment, we have undertaken this retrospective study in the southern part of Trivandrum district, Kerala, India. Out of the total 276 cases of wound infections from whom pus samples were collected and subjected to aerobic culture, 200 cases were culture positive (72.5%). Out of which 48 cases yielded more than 1 pathogen, up to 3. As a result total number of isolates was 250. The major pathogenic bacteria isolated were, Staphylococcus aureus (24.8%), Klebsiella spp (18%), Escherichia coli (15.2%), Pseudomonas spp (15.2%), Proteus spp (6.8%), Enterococcus spp (6.4%). Other less isolated species were Acinetobacter, Citrobacter, Enterobacter species, Streptococcus pyogenes, coagulase negative staphylococcus (CONS) and one fungal agent Candida spp. Out of the 62 Staphylococcus aureus strains isolated, 16.1% were methicillin resistant Staphylococcus aureus (MRSA). Among 60 Klebsiella species isolated, 25% were multi drug resistant (MDR) strains. Two strains of Pseudomonas species and 1 of Escherichia coli were also MDR. Among MSSA, 65.4% of the strains were resistant to Penicillin. On the other hand 100% of the strains were susceptible to Cloxacillin, Cefazolin, Cefuroxime, Linezolid and Vancomycin. Among MRSA, 100% resistance was noted against Cloxacillin, Ceforoxime, and Penicillin, with 100% sensitivity to Linezolid and Vancomicin. Of the 16 Enterococcus species isolated, 75% of the strains were resistant to Cotrimoxazole, followed by Ceftriaxone (68.8%) and Clindamycin (62.5%). Hundred % susceptibility was recorded against Chloramphenicol, Linezolid and Vancomycin. All the strains of Klebsiella isolated were intrinsically resistant to Ampicillin with appreciable resistance (64-71%) to cephalosporins, Cotrimoxazole, Amoxyclave and Cefaperazone/sulbactam. Escherichia coli isolates also shown similar pattern of reisistance. The resistance pattern of other strains are depicted under results. 
Keywords: MDR, MRSA, Klebsiella species, CONS, Wound infection, Pseudomonas, Proteus species