Determination of Antibiotic Residues in Raw Cow’s Milk Sold in two Dairies in the Koulikoro Region Using the HPLC Technique
Ousmane DIARRA1,2*, Youssouf KEITA1,3, Fanta Kaba CAMARA2, Boubacar Madio dit Aladiogo MAIGA3, Salimatou SAMAKE1,2, Aïssé BAH2, Mah FANE2, Fatoumata FARADJI2, Fassé SAMAKE1, 2 , Cheickna DAOU1,Mamadou WELE1,4, Amadou Hamadoun BABANA2 et al
1Institute of Applied Sciences – ISA University campus of Badalabougou, near the TOKTEN program, Bamako, Mali
2Research Laboratory in Microbiology and Microbial Biotechnology (LaboREM-Biotech), Faculty of Sciences and Techniques, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali
3Central Veterinary Laboratory (LCV), Bamako, Mali
4African Center of Excellence in Bioinformatics (ACE-B) University of Sciences, Techniques and Technologies of Bamako (USTTB), Mali.
Citation: DIARRA O, KEITA Y, CAMARA FK, MAIGA BM dit, SAMAKE S et al (2023) Determination of Antibiotic Residues in Raw Cow’s Milk Sold in two Dairies in the Koulikoro Region Using the HPLC Technique. SciTech Infectious Diseases 2023.
Received: October 27, 2023 Accepted: October 30, 2023 Published: October 30, 2023
Abstract
Veterinary treatments, mainly antibiotics, used for therapeutic or prophylactic purposes in dairy farming can be the cause of the presence of antibiotic residues in milk. However, these residues constitute a major health concern for consumers. For the dairy industry, whose objective is to have a raw material suitable for processing, it is necessary to screen for antibiotic residues in milk at each collection. This study is based on two parts, a survey of seventy people (10 practicing veterinarians and 60 milk producers) to describe the main molecules of antibiotics used in dairy cattle farming in the two basins of Tienfala and Kasséla in the Koulikoro region, and a search for residues by Delvotest SP with confirmation of positive samples by liquid chromatography (HPLC). To this end, sixty (60) samples of raw milk were collected in two sampling campaigns per site carried out 15 days apart. All samples were analyzed using the Delvotest SP rapid detection of antibiotic residues in milk kit first before being analyzed by liquid chromatography (HPLC) for positive samples. The results of the surveys revealed the predominance of three pathologies within dairy herds: diarrhoea, mastitis and pulmonary infections with a combination of several antibiotics belonging to five (5) different families of antibiotics. Of the 60 samples submitted for analysis, 38 were found to be positive with Delvotest SP, i.e. a contamination rate of around 63.33%. Of the 38 samples positive to the rapid test, 26 responded positively to confirmation by liquid chromatography, i.e. a rate of 68.42% with mainly four antibiotics belonging to the families of β-lactams and tetracyclines. The results of the study effectively prove the effective presence of antibiotic residues in raw cow's milk from the mini-dairies of Kassela and Tienfala. They thus characterize current practices in the treatment of dairy cattle with antibiotics in the farming areas studied. Compliance with withdrawal periods with the elimination of milk from treated cows must be obtained by raising the awareness of producers by animal health professionals.
Keywords: Antibiotic residues, HPLC, Raw milk, Bovine, Kassela Tienfala
