Galinsoga Parviflora Restored Associated Motor Coordination Through Increased Linear Distribution Of Purkinje Cells In Mercury Chloride-Induced Toxicity of Mice’s Cerebellum
John Tabakwot Ayuba1,2*, Akeem Ayodeji Okesina6, Ibe Michael Usman2, Michael Kunle Ajenikoko2, Theophilus Pius3, Nicholas Kusiima3, Saidi Odoma4, 5 and Mario Fernandez Edgar2
1Faculty of Medicine and Surgery, Mbarara University of Science and Technology, Mbarara, Uganda
2Department of Human Anatomy, Kampala International University, Ishaka-Bushenyi, Uganda
3Department of Medical Laboratory Science, Kampala International University, Ishaka-Bushenyi, Uganda
4Department of Pharmacology, School of Pharmacy, Kampala International University, Ishaka, Uganda.
5Department of Pharmacology, College of Health Sciences, Kogi State University, Anyigba, Nigeria.
6Department of Clinical Medicine and Community Health, Anatomy and physiology Unit, School of Health Sciences, College of Medicine and Health Sciences, University of Rwanda, Uganda.
Citation: Ayuba JT, Okesina AA, Usman IB, Ajenikoko MK, Pius T (2023) Galinsoga Parviflora Restored Associated Motor Coordination Through Increased Linear Distribution Of Purkinje Cells In Mercury Chloride-Induced Toxicity of Mice’s Cerebellum. SciTech Infectious Diseases 2023.
Received: November 08, 2023 Accepted: November 13, 2023 Published: November 13, 2023
Abstract
Mercury (Hg) is a poisonous substance associated with diseases, such as ataxia and Joubert syndrome. Therefore, it is important to find a way to disrupt the process of Hg poisoning in the cerebellum, by exploring the medicinal value of local herb such as Galinsoga parviflora (GP). This study examined the effects of aqueous leaf extract of GP in HgCl2-induced cerebellar toxicity in adult male mice. Twenty-five adult male mice of an average weight of 25g were randomly divided into 5 groups (n=5). Animals in Group I received oral administration of 2 ml/kg distilled water for 6 weeks, Group II received 2.3 mg/kg of HgCl2 for 3 weeks, Group III received 2.3 mg/kg of HgCl2 for the first 3 weeks followed by oral administration of 800mg/kg of GP extract for the next 3 weeks, Group IV received 800mg/kg of GP extract for the first 3 weeks followed by 2.3 mg/kg of HgCl2 for the next three weeks, and Group V received 2.3 mg/kg of HgCl2 and 800 mg/kg of GP extract concurrently for three weeks. The test animals were subjected to beam walking tests during the experiment period, followed by euthanasia, perfusion fixation, and tissue sample collection for histological and histochemical analysis. Treatment with the extract of GP showed varying degrees of regeneration in the cerebellar Purkinje cells and myelin sheath of mice in HgCl2 induced toxicity, with corresponding improvements in balance and posture. Cerebellar HgCl2 exposure in the present study was neurotoxic; however, treatment with GP was of therapeutic value.
Keywords: Mercury chloride, Cerebellum, Galinsoga parviflora
