International Conference on Biomedical and Cancer Research
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Accepted Abstracts

Sperm dynein ATPase activity and AAA1-2 expression : A regulation in asthenozoospermia

Silvia W Lestari*, Manggiasih, Dessy Noor Miati, Meidika Dara Rizki
Universitas Indonesia, Indonesia

Citation: Lestari SW,  Manggiasih,Miati DN, Rizki MD (2019) Sperm dynein ATPase activity and AAA1-2 expression : A regulation in asthenozoospermia. SciTech Bioemd-Cancer Scinece 2019. Tokyo: Japan 

Received: June 22, 2019         Accepted: June 26, 2019         Published: June 26, 2019

Abstract

Asthenozoospermia is a common cause of male infertility.  It is characterized by reduced sperm motility and has numerous cellular mechanisms. Unfortunately, there are restricted data on dynein ATPase and ATPase associated with various cellular activities (AAA) 1-2. This study explored the role of dynein ATPase activity and quantification of AAA1-2 dynein. Fifteen asthenozoospermia samples were used in this study. Semen analysis was conducted based on WHO 2010, while dynein ATPase was defined by the released inorganic phosphate and AAA was determined by ELISA. This study showed that the dynein ATPase activity in asthenoozoospermia was significantly lower than in the normozoospermia group (3.7 ± 0.3 vs 7.5 ± 0.4μmol Pi/mg protein/h, respectively, p<0.05). Furthermore, the quantification of AAA1 and AAA2 was showed unsignificantlylower in asthenozoospermia compared to normozoospermia group (1.7 ± 0.1 vs 4.5 ± 0.2; 3.8 ± 0.4 vs 5.6 ± 0.5ng/ml, respectively, p>0.05). The structure and function of damaged sperm dynein may alter dynein ATPase activity and levels of AAA1 and AAA2 in asthenozoospermia. 

Keywords: dynein ATPase; AAA1, AAA2, asthenozoospermia, male infertility