International Conference on Biomedical and Cancer Research
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Accepted Abstracts

Exploration of cervical-squamous-epithelial-cell (CSEC) biometrics from thinprep Pap smear on GA genotype of Fas-promoter-670 gene in Indonesian women living with highrisk HPV and Candida sp. Infection: A case report

Mauritius Lambertus Edy Parwanto1, Raditya Wratsangka1, Assangga Guyansyah1, Kirana Anggraeni1, Reza Aditya Digambiro1, David David1,Hanslavina Arkeman1, Hosea Jaya Edy2, Yosua Jaya Edy3,4, Sisca Sisca1, Haryo Ganeca Widyatama 1

  1. Universitas Trisakti, Indonesia
  2. Universitas Sam Ratulangi, Indonesia
  3. State Personnel Board The Republic of Indonesia, Indonesia.
  4. Universitas Kristen Indonesia, Indonesia
Citation: Edy Parwanto ML, Wratsangka R, Guyansyah A, Anggraeni K, Digambiro RA et al (2019) Exploration of cervical-squamous-epithelial-cell (CSEC) biometrics from thinprep Pap smear on GA genotype of Fas-promoter-670 gene in Indonesian women living with highrisk HPV and Candida sp. Infection: A case report. SciTech Biomed-Cancer Sciences 2019. Tokyo: Japan

Received: August 27, 2019         Accepted: August 29, 2019         Published: August 29, 2019

Abstract

Background: Human papilloma virus (HPV) infection can change cervical-squamous-epithelial cells (CSEC) to be abnormal. Epigenetically, this change in the direction of abnormality in CSEC is partly due to the polymorphism of the Fas-promoter-670 gene. High-risk HPV patients can be infected by other microbes, for example Candida sp. The purpose of this study was to show the appearance of Candida sp. and CSEC based on epigenetics on the Fas-promoter-670 gene polymorphism in Indonesian women with high-risk HPV infection. Biometric quantification is carried out with the following CSEC performance characteristics. Case Presentation: Indonesian women at the age of 28 years, living with high-risk HPV and Candida sp. infection, on April 21 2016 had a Pap smear examination using a thinprep method and also blood drawn from the cubital vein (vena cubiti). Blood and thinprep samples were detected of Fas-promoter-670 gene polymorphisms by restriction fragment length polymorphism (RFLP) with BstN1 enzyme. Epigenetically, the subjects in this case had the GA genotype of Fas-promoter-670 gene in both blood and thinprep samples. Candida sp. infection in patients is divided into early and advanced stages. Candida sp. infection in the early stages with characteristic by appearance of polymorphonuclear leukocytes on the hyfa, whereas at an advanced stage without polymorphonuclear leukocytes on the hyfa. CSEC performance (n cells=27) as follow: cell length (CL)=5.38±0.77 µm (CI 95%=5.10-5.67 µm), cell width (CW)=4.37 ± 0.57 μm (95% CI=4, 16-4.58 μm); cell area (CA)=20.29 ± 4.46 μm 2 (95% CI=18.62-22.01 μm 2 ); cell perimeter (CP)=16.52 ± 1.83 μm (95% CI=15.80-17.21 (μm); nucleus length (NL)=0.81 ± 0.11 μm (95% CI=0.77-0.86 μm); nucleus width (NW)=0.62 ± 0.09 μm (95% CI=95% CI 0.58-0.65 μm); nucleus area (NA)=0.41±0.10 μm 2 (95% CI=0,38-0.45 μm 2 ); nucleus perimeter (NP)=2.38 ± 0.27 μm (95% CI=2.29-2.49 μm); nucleus length index (NLI)=15.43 ± 3.03 (95% CI=14.31-16.62); nucleus width index (NWI)=14.46±3.38 (95% CI=13.32-15.78); nucleus area index (NAI)=2.16±0.75 (95% CI=1.90-2.45) and the nucleus perimeter index (NPI)=14.61±2.29 (95% CI=13.77-15.49). Conclusion: Epigenetically, the subject in this case had the GA genotype of the Fas-promoter-670 gene in blood and thinprep samples. We found the early stages of Candida sp. infection which is characterized by appearance of polymorphonuclear leukocytes on the hyfa, whereas at an advanced stage without polymorphonuclear leukocytes on the hyfa. Base on the result of biometrics characteristic such as CL, CW, CA, CP, NL, NW, NA, NP, NLI, NWI, NAI and the NPI, CSEC in this case is a normal. Our team recommends a re-examination after 6 months. Key words Epigenetically, Fas promoter-670 gene, Candida sp., cervical-squamous-epithelial-cells, cell biometrics