Phytomedicine has been a field of interest for explorative researchers. A wide variety of indigenous medicinal plants have been proved to possess exorbitant medicinal properties. One among these is Vernonia arborea, the tree species of the family Asteraceae. The decoction of the leaves and roots has been known to promote wound healing process, the roots are used to treat fever, the tonic from the leaves is taken by women after child birth and is said to have wound healing properties, the bark has been traditionally used to treat mouth ulcers. Adult Zebrafish wound models were established to check the healing efficacy of Vernonia arborea bioactive fractions. The model supported wound inflammation and regenerative studies. A bioactive fraction from the plant was found to be active against five tested wound pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli and Stenotrophomonas maltophilia.). Histopathological sections and biochemical analysis of the wound model treated with this bioactive fraction revealed that the fraction accelerated initial wound inflammation, enhanced early resolution of inflammation and induced cellular infiltration at the wound site resulting in reepithelialisation and early wound closure, all of which are characteristics to be possessed by a good wound healing agent. The ointment formulation of the bioactive fraction used for the study thus has a potent possibility to be used to treat wounds.