Adipose-derived stem cells (ASCs) resides in adipose tissue are considered highly promising for their abundancy, easy acquisition, and the therapeutic potential. Meanwhile, recent trends in regenerative therapy had shift from the cell transplantation to their derivates. ASCs-sourced secretome, comprises multiple biomolecules and extracellular vesicles, is therefore becoming a good candidate for stem cell-free therapy. Additionally, Hepatocyte growth factor (HGF) secreted by mesenchymal cells such as the ASCs, played a crucial role in the tissue regeneration of injured organs. Here, we firstly advocated that the amount of HGF as an indicator for evaluating the quality of ASC-conditioned medium (CM), one of the secretome derivates. The impact of culture environment as well as the components of culture media on HGF secretion was discussed.

Our in vitro analysis indicated that ASC-CM promoted the cell proliferation and the cell migration of HaCaT and NHDF (normal human dermal fibroblast) cells. Additionally, ASC-CM augmented HUVEC cells forming the networks on the matrigel, suggested a pro-angiogenesis effect of ASC-CM. Besides, ASC-CM has a critical role in regulating the inflammatory cytokines. Furthermore, we found that ASC-CM accelerated the wound healing progress in diabetic mice. Our findings suggest that ASC-CM hold great potential for therapeutic use in regenerative medicine.