The revolution in the best growth and high yield of plants and crops is due to genetic modification. We explain a simple and efficient method for the genomic DNA extraction from citrus woody plants. Harvesting of fresh leaves from orange plants is done as it contains low level of poly phenyl. DNA extraction is relatively easy  from citrus woody than non-citrus plants. CTAB is used in this method of DNA extraction. Ethanol and NaCl are used to remove plants polysaccharides. Same volume of isopropanol is added into supernatant to make DNA pellet. Use 70% ethanol to wash DNA pellet. Then pellet dissolve in TE buffer. 50-500micro g/g DNA pellet is used as a sample. Then PCR & RFLP analysis are carried out. This DNA then can be stored for long durations. Changes and modifications can also be done to get the best quality and high yield from woody plants as well as all kinds of crops.

 

Keywords: CTAB, DNA pellet; Genomic DNA extraction, PCR, RFLP, TE buffer