Effect of Iron Fortificants & Prebiotics on RBC Count, WBC Count and Platelets in Iron Deficient Female Sprague Dawley Rats
Abdul Momin Rizwan Ahmad1*, Waqas Ahmed2, Sanaullah Iqbal2, Muhammad Hassan Mushtaq2, Riffat Aysha Anis3, Umar Farooq3
1National University of Medical Sciences, Pakistan
2University of Veterinary & Animal Sciences, Pakistan
3The University of Lahore, Pakistan
Citation: Ahmad AMR, Ahmed W, Iqbal S, Mushtaq MH, Anis RA et al (2021) Effect of Iron Fortificants & Prebiotics on RBC Count, WBC Count and Platelets in Iron Deficient Female Sprague Dawley Rats. SciTech Biomed-Pharma Sciences 2021.
Received: August 09, 2021 Accepted: August 11, 2021 Published: August 11, 2021
Abstract
The present research was designed to determine if prebiotics and iron fortificants had some effect on RBC count, WBC count and Platelets. For this study, n = 126 female Sprague Dawley rats aged 6 to 8 weeks were obtained from the National Institute of Health, Islamabad. Two prebiotics and two iron salts were used in varying dosages to prepare various types of feed to be fed to rats. Initially, anemia was induced among rats by feeding them with carbon tetrachloride which is an iron binder. After that, rats were orally fed with fortified feed daily for a period of three months. Blood samples of overnight fasted rats were collected at 0, 30th, 60th and 90th day of the trials. RBC count, WBC count and Platelets levels were determined using the standard procedures.
Results: The study results indicated that mean squares for RBC count were significantly different with regards to the effect of groups, study intervals as well as their interaction. However, there was no effect of prebiotics and iron fortificants on WBC count and Platelets of iron deficient female Sprague Dawley rats. Conclusion: The study concluded that iron and prebiotic fortified feed improved RBC count but did not have any effect on WBC count and Platelets.
Keywords: Prebiotics, Iron Fortificants, RBC count, WBC count, Platelets, Iron Deficiency