Aim: To present options for management of errors resulting from the cryopreservation of large volumes of semen samples and long freeze to thaw intervals.
Background: The cryopreservation of gametes is on the rise owing to its established safety and efficiency.
Case description: We present a challenging case at our center of a lost semen vial due to a broken cryocane and a systematic approach to locating it.
Conclusion: Systematic troubleshooting can help locating misplaced gamete samples in high volume centers.
Clinical significance: New guidelines for containment systems is the need of the hour.
Keywords: Case report, Cryopreservation, Cryotanks, Liquid nitrogen, Cryocane, Cryovials