Air pollution is the main problem in the world, the physical, chemical and biological changes of air is called pollution. The major component in air pollution is carbon dioxide (CO2) produced through the burning of petrol, diesel, coal and biomass. The air pollution causes several respiratory and heart diseases along with cancer in the animals. Scientist recently it found that air pollution alters the antibiotic tolerance of bacteria. In our experiments bacteria isolated form polluted air in traffic roads of Anantapur city in India, by plate exposure methods, in day at 6-8 am, 1-3 pm and 6 -8 pm during summer, raining and winter seasons. The average bacteria were identified as 6.2× 102 (6-8 am), 8.2× 102 (1-3 pm) and 8.5× 102 (6-8 pm) CFU/m3 indicated that the polluted air contaminated with bacteria. Microscopic, biochemical and molecular characters was studied for selective isolates form the air samples. The majority of the isolates were identified as Gram positive Streptococcus spp, Staphylococcus spp and some gram negative Pseudomonas spp. The antimicrobial susceptibility test was performed by using disc and broth dilution methods. The Streptococcus spp, Staphylococcus spp and Pseudomonas spp showed high resistance to β lactam antibiotics such as Penicillin, Ampicillin and Amoxicillin.