Carbimazole administration, in excess, is associated with various pathological conditions, which includes testicular damage. Generation of oxidative stress is one of the plausible mechanisms of carbimazole-induced testicular damage. The aim of the present study was to investigate the effects of carbimazole on testicular function in male albino rats, as well as assess the ameliorative role of the combination of Vitamin C and tomato extracts treatments. Phytochemical analyses of the tomato extracts were performed. A total of 25 male albino rats weighing 200-250 g were randomly divided into five groups (I-V), with five rats in each group.
Group I served as normal control and received no treatment. Group II served as the negative control and received only carbimazole (60 mg/kg, oral). Groups III, IV and V served as the treatment groups. They all received carbimazole, and then Vitamin C only (200 mg/kg, oral), tomato extract only (30 mg/kg, oral), and vitamin C plus tomato extract respectively for 3 weeks. Oral administration of carbimazole (60 mg/kg) for 3 weeks resulted in the reduction of serum testosterone level in the negative control rats (group II) from a mean baseline of 0.05±0.00 ng/dl to 0.03±0.00 ng/dl. Histopathological results concomitantly revealed severely eroded seminiferous epithelia and increased interstitial space in this group. However, groups that received daily doses of Vitamin C or tomato extracts alone, or a combination of both, showed attenuated testicular damage, as evidenced by their testosterone levels which differed significantly compared to the negative control (P<0.05): 0.05±0.01 ng/dl, 0.09±0.07 ng/dl and 0.04±0.00 ng/dl) for groups III, IV and V respectively. Therefore, a combination of Vitamin C and tomato extract has protective effects against carbimazole-induced testicular damage.
Key word: Carbimazole, Vitamin C, tomato, medicinal food, testiculoprotective ethnopharmacology