Purpose: To investigate the In-Vivo Anticancer and Immunostimulatory Activity of n-butanolic extract of leaves of Mikania micrantha and its isolated compound 5,7 di hydroxy 6 methoxy 2 phenyl chromen 4- one.
Methods: The isolation of the compound was done by Flash Chromatography and the isolated compound was characterized through UV, IR, NMR and Mass data. The Anticancer activity was performed on EAC cell line in Swiss albino mice. The activity was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological and histopathological parameters of EAC bearing animals. The Immunostimulatory activity was performed through carbon clearance, Delayed Type Hypersensivity (DTH), neutrophil adhesion and Humoral Antibody (HA) titer methods.
Results: At the dose of 500 and 1000 mg/kg/day p.o for the extracts and 2.5 mg/kg/day p.o. for the isolated compound, significantly decrease the tumor volume (3.09 ± 0.027 ml, 2.66 ± 0.165ml and 1.93±0.012), increased the life span (58.89%, 66.39% and 75.24%) and significantly (P<0.05) decreased tumor weight as compared with control. Hematological profiles were found to be nearly normal level in extract treated mice compared with tumor bearing control mice. The Immunostimulatory Activity was also found to be effective in the above dosage regimen. The results revealed that animals treated with above doses show significant increase in rate of carbon clearance from blood, increase in HA titer value, increase in neutrophil adhesion and significant (P<0.05) increase in mean paw edema in delayed type hypersensitivity reactions in dose dependent manner.
Conclusion: The results demonstrated that the extract possessing dose dependent anticancer activity and immunostimulatory activity attributed to the presence of flavonoidal compounds.
Keywords: HA titer, EAC cell line, LD50,, DTH, BUT, M. micrantha