Parkinson's disease (PD) is a neurodegenerative disorder of the central nervous system. It results from the death of dopamine generating cells in the substantia–nigra, a region the mid brain, the cause of the cell death is unknown. Isatin derivatives shows maximum anti-Parkinsonian activity, In – Vitro and In – Vivo anti-oxidant activity. Schiff bases of Isatin derivatives were investigated for In-Vitro antioxidant activity. The following tests such as Nitric oxide scavenging activity, DPPH scavenging activity and acute oral toxicity studies were performed and investigated for Anti- Parkinson’s activity. The following tests such as, Assessment of behavioral and biochemical parameters, Determination of acetylcholine levels, Determination of Acetyl cholinesterase activity were performed and also investigated for In-Vivo antioxidant activity. Estimation of superoxide Dismutase, estimation of Lipid Peroxidation, Estimation of reduced glutathione levels were performed. Isatin derivative test compounds when given at a dose of 30mg/kg body weight administered orally to rat they showed maximum anti Parkinsonian activity, decreased tremors, salivation and lacrimation.

 Keywords: Isatin, Anti-Parkinson’s activity, In – Vitro, In – Vivo anti-oxidant activity