Alzheimer’s disease (AD) is an irreversible, progressive brain disorder that slowly destroys memory and thinking skills and eventually the ability to carry out the simplest tasks The present investigation was undertaken to evaluate anti-Alzheimer’s  activity of novel herbal aerosol spray formulation containing pure extracts  of  Curcumin, Guggul and Withania somnifera. A novel aerosol spray was formulated using spay dried polyherbal dispersions  F-1, F-2  and F-3 (1:1, 1:2 and 1:3 ratios each) and their quality control, stability and efficacy studies was carried out as per ICH and OECD guidelines respectively. In this study formulation of polyherbal spray dried particles using chitosan as carrier and characterization of spray dried formulations were performed. Pressurized aerosol packs containing polyherbal spray dried dispersions in the concentration range of 0.5, 1 and 2% w/v dispersed in 10/90, 20/80, 30/70/, 40/60, 50/50) propellant blends of   HFA-134  were developed in 3 in 1 aerosol filling machine and evaluated. Accelerated stability studies of aerosol formulations were assessed. Behavioural studies were performed in male wistar rats and histopathologicl studies were performed. The preparations were also assessed for AChE inhibitory activity by the Ellman method. Results with P<0.05 was considered statistically significant. The current study is the first study that proved the research evidence for the possible positive effects of the aerosol formulation of spray dried polyherbal extracts on cognitive performance, learning and memory function in the haloperidol induced Alzheimer’s disease model in male wistar rats.

Keywords: Alzhiemer’s, Herbal, Spray dried chitosan, Aerosol nasal spray