Surgical wounds swab culture and antibiogram is one of the most important methods of confirming post surgical wound infections, health problems aggravated by the invasion of wound pathogens and investigating the pace of development of multidrug resistance surgical wound isolates. This research was aimed at detecting the proximate bacteria associated with postsurgical wounds with clinical signs of infection of patients attending Ibrahim Badamasi Babangida specialist hospital Minna, Niger state, North central, Nigeria. Hundred wound swabs sample were aseptically collected from infected post surgical wounds of patients for the duration of 4 months. All the samples were immediately taken to the laboratory where they were all cultured aerobically on sterile Nutrient agar, Blood agar, MacConkey agar Manitol salt agar, Oxacillin Resistance Screening Agar (ORSA) and Cetrimide agar for 24 hours. The bacteria colonies on the plates were identified using standard microbiological procedures such as Gram staining and biochemical reactions. However, antibiogram of all the isolates were performed by Kirby Bauer’s disc diffusion technique using commercially formulated multidisc antibiotics on Muller Hinton agar and interpreted as recommended by Clinical Laboratory Standard Institute guidelines. PCR technology was used to confirm the Methicillin Resistance Staphylococcus aureus strain. The results from this study showed growth of 68 (68%) out of the 100 samples inoculated. Seven different species of bacteria were isolated 3 of which were Gram positive (ie 24 S. aureus isolates, 4 Streptococcus pyogenes isolates and 3 Streptococcus agalactiae isolates) and four were Gram negative (16 pseudomonas sp isolates, 11 E coli isolates, 6 Proteus mirabilis isolates and 4 Klebsiella sp isolates). Furthermore, the result of the antibiogram revealed that 19 (79.2 %) of the S aureus were sensitive to both Amoxicillin and Ciprofloxacin. However, 13 (54.2%) of the isolate were resistant to Gentamicin, Ampiclox and Zinnacef. Meanwhile, all the 3 Streptococcus agalactiae isolates were sensitive to Ampiclox, Amoxicillin, Ciprofloxacin, Streptomycin, Septrin and Erythromycin, but, 2 (66.7%) Streptococcus agalactiae isolate were resistant to Gentamicin and Zinnacef. More so, 3 (75%) of Streptococcus pyogenes isolates were sensitive to both Ampiclox and Erythromycin, while 2 (50%) Streptococci pyogenes isolate were resistant to Ciprofloxacin, Streptomycin and Septrin. Also, it was observed that 14 (87.5%) Pseudomonas sp isolates were sensitive to Nalidixic Acid. Whereas, 10 (62.5%) were resistant to Ciprofloxacin. In addition, 90.9% of Escherichia coli isolated in this study were sensitive to Septrin. But, 72.2% (8) of Escherichia coli isolate were resistant to Ceporex. The result of this study also revealed that all the 6 (100%) Proteus mirabilis were sensitive to both Gentamicin and Streptomycin and none of the Klebsiella sp were resistance to Tarivid, Peflacine, and Ciprofloxacin. The agarose gel electrophoresis of the amplified MecA gene of Methicillin Resistance Staphylococcus aureus isolates showed the presence of MecA gene and band was at 310bp. Based on the findings of this study, the antibiogram of the bacteria revealed that the surgical wounds used in this study were infected with multidrug resistant bacteria, hence draws great attention to multidrug resistance bacteria in IBBSH.
Keywords: Surgical wound, electrophoresis, antibiotic, sensitivity disc, MecA gene.