World Congress on Immunology & Microbiology
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Accepted Abstracts

Effects of Lactobacillus spp. isolated from the sap of palm tree Elaeis guineensis (palm wine) on cellular and innate immunity

Eze Christopher Osita*, Berebon Dinebari Philip, Gugu Thadeus Harrison, NworuChukwuemeka Sylvester and Esimone Charles Okechukwu
University of Nigeria Nsukka,Nigeria

Citation: Osita EC, Philip BD, Harrison GT, Sylvester NC, Okechukwu EC (2019) Effects of Lactobacillus spp. isolated from the sap of palm tree Elaeis guineensis (palm wine) on cellular and innate immunity. SciTech Immuno-Microbiology 2019. Dubai: UAE

Received: February 06, 2019         Accepted: February 07, 2019         Published: February 07, 2019

Abstract

The isolation, characterization and effects of lactobacillus spp. from fresh palm wine was undertaken in this study. The aim of this study was to isolate and investigate the in vivo effects of the isolates on innate and cellular immune system. The result of the phenotypic and genotypic characterizations showed the presence of Lactobacillus brevis, Lactobacillus paracasei subsp. Tolerans, Lactobacillus paracasei and Lactobacillus yonginensis, which are members of Lactobacillus spp., isolated. The effects of the isolates on innate and cellular immunity were investigated, using two models namely: in vivo leukocytes mobilization rate (LMR) and delayed type hypersensitivity response (DTHR). The different isolates had very significant effect on cellular immunity as represented by their ability to stimulate delayed type hypersensitivity response in treated rats. They caused a percentage increase of 300, 600, 600, 500 and 650 for L. brevis, L. paracasei subsp. Tolerans, L. paracasei, L. yonginensis and positive control respectively when compared with the negative control. Similarly, the various isolates also affected significantly the innate immune system through their marked influence on the total leukocyte count according to the result of the experiment. They were able to produce a percentage increase of 213, 204, 188.6, and 152.5% for L. paracasei, L. yonginensis, L. paracasei subsp. Tolerans and L. brevis respectively; while the positive control produced 83.8% increase when compared with the negative control. The result of this study showed that the four lactobacillus spp. isolated from fresh palm wine significantly affected the innate and cellular component of the immune system positively.

Keywords: Delayed type, Hypersensitivity response, Innate immunity, Cellular immunity, Leukocyte mobilization.