The effect of pretreatment with toll-like receptor 4 antagonist resatorvid on methotrexate-induced liver injury in rats: Histopathological study
Bassim I Mohammad1, Bassim S Ahmed2, Alaa F Hassan3*, Samer F Hassan4
1 University of Al-Qadisiyah, Iraq
2 Mustansiriyah University, Iraq
3 Al-Mahmoudiya General Hospital, Iraq
4 Wasit University, IraqCitation:
Mohammad BI, Ahmed BS, Hassan AF, Hassan SF (2019) The effect of pretreatment with toll-like receptor 4 antagonist resatorvid on methotrexate-induced liver injury in rats: histopathological study. SciTech Immuno-Microbiology 2019. Dubai: UAE
Received: March 18, 2019 Accepted: March 18, 2019 Published: March 19, 2019
Objective: This research aims to evaluate the histopathological changes after pretreatment with resatorvid against methotrexate induced-liver injury.
Methods: 28 male albino-wistar rats divided into random 4 groups (7 rats in each). Control group: Rats left untreated. Vehicle pre-treated group: Rats were administered dimethyl sulfoxide (DMSO) followed by methotrexate (MTX). Methotrexate treated group: Rats left untreated then administered MTX. Resatorvid pre-treated group: Rats were administered resatorvid followed by MTX. 24 h after the end of treatment, the animals were sacrificed. Liver tissue samples dissected out immediately and fixed in 10% formalin. The traditional procedures (paraffin-embedded method) was used to prepare liver tissue for microscopic evaluation by none alcoholic fatty liver disease (NAFLD) Activity Score Components.
Results: Liver tissue sections of MTX-treated group show moderate-to-severe steatosis of hepatic cells and micro- and macro- hepatocellular fatty degeneration and giant fatty cysts with chronic inflammatory cells infiltration. While liver tissue sections of the resatorvid pre-treated group show moderate hepatic cellular fatty degeneration, with a decreased number of fatty cysts chains and the inflammation disappeared.
Conclusion: Resatorvid hepatoprotective effect against MTX-induced injury was promising throughout resolving the accompanying inflammation and partial restoring histopathological fatty alterations.
Keywords: Liver steatosis, Methotrexate sodium, Resatorvid, TLR4 receptor.