Objective: To Identify molecular characterization of swine flu (H1N1) pdm 09 and seasonalflu(influenza A).

Materials and Methods: NP/OP Swab specimen of 142 patients were collected aseptically in VTM. Nucleic Acid was extracted manually and was processed in Real-Time PCR foridentification of swine flu (H1N1) 09 pdm and seasonal flu (inf A).

Results: In this study, 142 patients presented with signs and symptoms of Flu like illness patients and were tested by Real-time PCR. Out of total 32 (22.53%) positive specimens, 18(56.25%) were positive swine flu(H1N1) 09 pdm and 14(43.73%) were positive seasonalflu (inf A).

Conclusion: We report a tiny outbreak of 18 swine flu(H1N1) 09 pdm and 14 seasonal flucases in our hospital, from Jan 2022 to June 2022. The outbreak involved persons of all agegroups, but it mostly affected paediatric age group. An outbreak of seasonal influenza occursdue to antigenic drift, which arises from point mutations in the viral genome. The mutationslead to the development of new strains of the flu strain that are immune to previous strains. Itis possible for influenza viruses with novel HA and/or NA genes to develop from geneticassortment among human, swine, and/or avian influenza strains.

Keywords: H1N1 Virus, Real-Time PCR, Seasonal flu, Swine flu