Aims and Objectives: To determine the microbiologic pattern of seminal fluid in male infertility.

Subjects and Methods: A retrospective laboratory review of the microbiologic analysis of 360 semen samples done in Benue State University Teaching Hospital, Makurdi, from January 2013 to January 2016.

Results: Fifty-one percent (N=184/360) of the semen samples were normozoospermic, 43% oligospermia (with 3%severe oligospermia), and 6% azoospermic. Sixty percent (N=216/360) of the semen samples contained sperm cells that were progressively motile, 30% non-progressive, and 10% non-motile. Ninepercent of the semen samples contained dead sperms (necrospermic). Forty–two percent (N=150/360) of thesemen samples were infected (contained a significant number of pus cells, ≥ 2000 cells per ml). Pathogens were isolated in 47%(N=70/150) of the infected semen samples as follows, Staphylococcus aureus (53%), Staphylococcussaprophyticus (10%), Escherichia coli (11.4%), Klebsiellaspp (7.1%), Streptococcus pneumonia (4.4%),Pseudomonas aeruginosa (7.1%) and Candida spp (7.1%). Moxifloxacin was 85% effective, Levofloxacin (80%), Ceftazidime (80%), Ceftriaxone(80%),Ciprofloxacin (70%), Ofloxacin (68%) and Gentamicin (60%). While Penicillin(20%) and Tetracycline (10%) showed poor activity.

Conclusion: Regular semen microbiologic investigation is quite invaluable to male infertility intervention in poor resource countries.

Keywords: Male infertility, Microscopy, Culture, Semen