Physical stimuli hold great impact on bodily functions. Their recognition for use as a therapeutic modality is limited due to lack of scientific evidence. Low arousal sounds have been shown to modulate neurophysiological processes; however, there remains a lack of data on neurochemical and histological findings. We have explored the neurochemical, electrophysiological, histological and molecular responses after exposure to low arousal sounds. Therapeutic sounds have revealed significant effects in reducing anxious behaviors in mice, reflected in increased time spent in open arms of elevated plus maze. Also, it has shown increased exploratory behavior in open field test. Neurochemical correlates have shown enhanced dopamine in treatment groups and increased corticosterone levels in brain. Histological analysis has revealed healthy parenchymal markers following stress induction and therapeutic sound treatment. Studies on the effects of low arousal sound has revealed enhancement of alpha rhythm in the right frontal cortex, increased RMSSD and  HF component of heart-rate variability, significant reduction in stress index and significant enhancement in heart-beat evoked potentials, reflective of enhanced interoceptive processing of cardiac signals, thereby reflecting improvement in heart-brain connection. These results provide an insight on the therapeutic mechanisms of sound-based therapies, which suggest that sounds hold potential to be used as a therapeutic modality.