World Summit on Immunology, Microbiology & Infectious Diseases
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Accepted Abstracts

Sexual Transmission of American Trypanosomiasis in Humans: - Insights in to Evolution and Pathology in Chagas Disease

Antonio RL Teixeira*
University of Brasília, Brazil

Citation: Teixeira ARL (2020) Sexual Transmission of American Trypanosomiasis in Humans: - Insights in to Evolution and Pathology in Chagas Disease. SciTech Immuno-Microbiology 2020. 

Received: August 31, 2020         Accepted: September 03, 2020         Published: September 03, 2020

Abstract

Scope: The long-lasting asymptomatic American Trypanosomiasismay translate into Chagas disease (CD). The Trypanosoma cruzi (Tc) infections spread across fiveContinents due to human population mobility, and CD is the most prevalent cause of heart failure in the Western Hemisphere. The hypothesis that Tc can be sexually transmitted was investigated.
 
Experiments: The blood and the semen samples were secured from 109 study subjects in four families. The Tc infections were assessed through the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence (IF) of the Tc antibody. The protozoan nuclear (nDNA) and the mitochondrial DNA (kDNA) were assessed by the PCR with specific primer sets. All these experiments were conducted in three occasions one year apart.
 
Findings: The ELISA and the IF showed the Tc antibody in 31/109 (28.4%) families’ members. However, the nDNA-PCR confirmed by Southern hybridization, cloning and sequence revealed positive results in 83/109 (76%) family members. The instills of nDNA-PCR+ semen aliquots in the vagina or in the peritoneal cavity of mice resulted into Tc nests in the somatic cells and in the organs ofreproduction. The absence of the Tc antibody in 62.5% of the families’ members explained by the vertical transmission of Tc infections, and immune tolerance shownin chicks hatched from Tc-infected eggs. The kDNA-PCR+results in 91/109 (83%) explained by the vertical transfer of the mitochondrial kDNAsequences into the host’s genome. Multiple kDNA mutations translated into Chagas-like heart disease.
 
Conclusion: The sexual transmission of Tc in humans is a pandemic route for the genetically driven Chagas disease and biodiversity.