Coeliac disease arises from immunological sensitization of small intestine to dietary gliadin and is associated with HLA.DQ2 ,HLA.DQ8 on chromosome 6 ,environmental factors are also important in the developmental of overt disease and possibly other non-HLA associated genes. Patients who cannot secrete ABO and Lewis blood group antigens into body fluids , an ability controlled by a single gene on chromosome 19, are known to be at increased risk of bacterial ,fungal ,viral ,allergic conditions, gastrointestinal diseases ,malignancy, and some autoimmune disorders associated with human leucocyte antigen (HLA) markers. According to blood type diet theory, blood group O should avoid grains and blood group A  thrive well as vegetarians. As secretor state is closely associated with expression of Lewis antigens both in secretions and on leucocytes, determination of red cell Lewis phenotype provide a simple method for measuring the prevalence on non-secretors within certain population with specific disorders. We screened thirty one known Coeliac patients  on GFD and 27 age and gender matched control for major and minor blood groups using gel electrophoresis by (Bio Rad Laboratories ,Dia Med ,Switzerland),in central Blood bank in the university hospital-Tripoli. We conclude that determination of major blood groups alone did not contribute to the susceptibility of Coeliac disease. None of the control in this study was Le( a+b-), where Coeliac patients account for 52% of group O Le (a+b-) and 41.7% of blood group A Le(a+b-).  Although JK(a-b-) is rare in most population, it is very common in Libyans (66.7%  in control to 93.5% in Coeliac patients ), the increased risk of Coeliac disease in  JK(a-b-) ,and blood group A  JK(a-b-) p=0.003, to our knowledge  has not been reported  before and needs further evaluation.