In the present work, Nano-mechanical properties of intermetallic layer formed on 316L austenitic stainless steel have been investigated. Al-Si alloys with varying percentage of silicon i.e. 8%, 10%, 12%, and 14%, using hot-dip method. Nano-mechanical properties of the Intermetallic  formed  have  been  evaluated  using  Nano-indentation  technique   at   room   temp era tu re and results were compared. On the basis of our observations and obtained results it is concluded that with increasing silicon percentage the thickness of the intermetallic layer increases. Howev er, the Na no-mechanical results showed that this layer is brittle in nature. Therefore, it can be inferred th at for bio-implant application the thickness of the lay er needs to be at lower level as possible.