Antibiotics are a class of naturally-occurring and synthetic chemical compounds with antimicrobial activity and are widely used in human and veterinary medicine. But regrettably this is not the case as evidenced in recent years. Although the antibiotics have improved the health of countless numbers of humans and animals, many antibiotics have also been losing their effectiveness  

The resistance among various pathogens to different antimicrobial drugs has emerged as a cause of public health threat all over the world at a terrifying rate. Considering the adverse effects of MDR, the present study, emphasizes the problems associated with MDR and the need to understand its significance and mechanisms to combat microbial infections. Characterization of all isolates gave surprising results showing the emergence of Staphylococcus aureus are resistance to Penicillin and Methicillin. They were tested for the antimicrobial activity by using fungal extracts where the action of the fungal extracts were determined by FT-IR. FT-IR Analysis for  mass of different wavelengths confirms the presence of major fuctional groups like Amines, Alcholos, Ester, Aldehyde, Chloride compounds in the Penicillium sample and it confirms the presence of major fuctional groups like Primary and secondary amines, Alkanes, Amines, Ester, Aromatic compounds in the Trichoderma sample.

Our study highlights that the multiple drug resistance development in these hospital bacterial micro flora is directly related to lack of improper disposal methods which could be the result of misuse and repeated use of antibiotics. The results suggest that the multi-drug resistant Staphylococcus aureus from the Hospital effluents and sewage may act as a possible source of transfer of these highly resistant pathogens and their genes to human that could be threat for the treatment of disease by commercially available antibiotics.

Keywords: Multi Drug Resistance, Staphylococcus aureus, hospital effluents