Properties of Glucose Oxidase produced from a newly isolated strain of Aspergillus niger
Onosakponome Iruoghene *1Okoye Ifeanyi2, Awhin Prosper Ejiro3, Ezugwu Arinze Linus4, Ajoh Alfred Ikechukwu5, Chilaka Ferdinand Chiemeka6
1,Dennis Osadebay University, Anwai, Asaba, Delta State, Nigeria
2,4,6 University of Nigeria, Nsukka, Enugu State, Nigeria
3,5Delta State University, Abraka, Delta State, Nigeria
Citation; Iruoghene C, Ifeanyi O, Ejiro AP, Linus EA, Ikechukwu AA (2023) Properties of Glucose Oxidase produced from a newly isolated strain of Aspergillus niger. SciTech BioTech-Food Sciences 2023.
Received: October 16, 2023 Accepted: October 20, 2023 Published: October 20, 2023
Abstract
Glucose oxidase (GOx) is a key enzyme used in many industries worldwide. To identify the GOx producer strain, Aspergillus niger was isolated from the Beehive and screened for its glucose oxidase-producing capability. Glucose oxidase was mass-produced by a submerged fermentation system. The activity of glucose oxidase was ascertained by the continuous spectrophotometric rate determination method. GOx was purified by (70%) ammonium sulphate precipitation, dialyzed, and gel filtration with 2.82, 3.54, and 3.69U/mg specific activity respectively. The enzyme exhibited an optimum pH range of 5.5 and a temperature optimum of 40°C. Ag2+ and Hg2+ had a remarkably inhibitory effect on the partially purified GOx, whereas Ca2+, Zn2+, and Mg2+ do not affect the enzyme activity. Cu 2+ and Co2+ have a slight inhibitory effect on GOx. Kinetic characteristics of GOx from Aspergillus niger display Vmax as 40 U/ml, and Km as 0.12 mM. The findings on the properties of Gox exhibited optimum conditions for industrial applications
Keywords: Aspergillus niger, Glucose oxidase, Characterization, Purification, Bee hive
