Introduction: Akhizunber, a novel herbal preparation delivered for the treatment of oral mucosal ulcers, was prepared from the medicinal herbs of Achillea asiatica Serg, leaves of Juniperus sabina L, and roots of Bergenia crassifolia (L) Fritsch in a ratio of 2:1:2, immersed for 1 week in 20% ethanol. For those reasons, we aimed to study effect of Akhizunber in the treatment of oral candidiasis.
Goal: To study Akhizunber in the treatment of oral fungal infections.
Materials and methods: The in vitro method of analysis is based on the methodology of Department of Microbiology, Tokushima University. A total of 50 patients attended to the Dental Hospital of the School of Dentistry, Mongolian National University of Medical Sciences and diagnosed with oral fungal infection were participated in this study. After clinical assessment and diagnostics has been made, differentiation of Candida species and testing of their resistance to antifungal agents were tested. In experimental group oral administration of azole antifungal agents with local treatment with Akhizunber was performed. 
Results: Killing assay - Akhizunber did not effect on the growth of C. Albicans. Anti-biofilm activity of Akhizunber on biofilm formation was statistical significance (p<0.01). The effect of 1% and 2.5% Akhizunber reduced initial attachment (p<0.05) of C.albicans. The 5%, 10% and 20% Akhizunber inhibit hyphae formation from yeast. SEM- Biofilm cells on cell desks were clearly decreased by Akhizunber. The prevalent fungal species isolated from the oral lesions was C.albicans (84%). Antimycotic resistance tests revealed the high antymicotic activity of Fluconazole and Flucytosine against causative agents. After local treatment of oral lesions with Akhizunber together with oral administration of antifungal agent full clinical cure was observed on 3^rd-5^th day of therapy in patients with acute candidiasis, and on 3-5^th day in patients with chronic manifestations.
Conclusion: Akhizunber preparations inhibit to growth of Candida albicans, especially on hyphae form. Oral administration of antifungal agent in combination with the local treatment of oral lesions with Akhizunber in the combind therapy of patients with oral candidiasis showed high effectiveness.
Keywords: Candida albicans, Akhizunber, Povidone iodine, Oral candidiasis