Objectives: This study was aimed at evaluating the anti-diabetic activities of Zizyphus spina-christi seeds embryos extract (ZSC seeds embryos extract) on general characteristics of diabetes, carbohydrate metabolism enzymes and lipids profile in alloxan-induced diabetic rats. Results: A single injection of alloxan to rats (150mg/kg b.w) caused pathological alterations in all studied parameters and histological structure of the pancreas. Treatment of diabetic rats with ZSC seeds embryos extract normalized this alterations. Diabetic rats were treated with ZSC seeds embryos extract (2doses; 200&400 mg/kg b.w) and glibenclamide (0.6 mg/kg b.w) for 6 weeks. Fasting blood glucose (FBG) levels were measured on zero time, 4 h, 8 h and 24 h. Also serum glucose level and body weight were measured every week for all rats. Results showed that oral administration of ZSC seeds embryos extract caused significant reduction in blood glucose level and plasma lactate together with significant elevation in serum insulin, serum pyruvate with normalization in body weight. Marked elevation in total antioxidant capacity (TAC) with lowering of percentage of glycated haemoglobin (HbA1C %) was reported. In line with amelioration of the diabetic state, ZSC seeds embryos extract restored hepatic and muscle glycogen content and hexokinase activity together with significant decrease of glucose-6-phosphatase and fructose 1, 6 biphosphatase enzymes activity. Moreover, the extract succeeded to reduce the elevated serum total cholesterol, triglyceride (TG) and low density lipoprotein (LDL) levels and to elevate the reduced high density lipoprotein (HDL) level of diabetic rats. Conclusion: This study for the first time reveals that ZSC seeds embryos extract, showed marked amelioration in diabetic rats by stimulating of insulin release from the remnant β-cells, inhibiting glucagon secretion from alpha-cells and increase of antioxidant mechanisms, as well as attenuation of meal-derived glucose absorption. This may be attributed to the presence of saponin glycosides, polyphenols, flavonoids and terpenoids which phytochemical screening revealed its presences in ZSC seeds embryos extract in this study. Keywords: Zizyphus spina-christi Seeds Embryos, Diabetes, Carbohydrate Metabolism Enzymes, Lipids Profile