Severe acute respiratory syndrome coronavirus2 caused a pandemic disease called COVID-19.The COVID-19 appeared in China in 2019 Egypt become infected on Feb 2020 with SARS-CoV2. The pandemic virus consists of a positive single strand ssRNA. The spike protein of the virus is responsible for virus attachment with cell receptor angiotensin converting enzyme two (ACE2).The genome of SARS-CoV2 consists of two major portions.

The samples were taken from patients with COVID-19 symptoms at military hospital in Egypt and transported to the main chemical laboratories under all the biosafety measures according to WHO guidelines. All samples were tested with RT-PCR. Positive samples were cultured using VeroE6 cell lines. The propagated virus was isolated and inactivated .The isolated virus was sequenced using next generation sequencing and submitted into gene bank . This study can be referenced for researchers in Egypt who aimed to propagate and isolate SARS-CoV-2 to work in vaccine production, genomics, sequencing, proteomics, gene editing like CRISPR and anti-viral drug screening.

 

Key words: RT-PCR; SARS-CoV-2; COVID 19; VeroE6; Inactivated Vaccine.