Objective: The Present study was focused on determining the antitumor potential of Hinokitiolon diethyl nitrosamine (DEN) induced Hepatocarcinogenesis in rat models via the inflammatory mechanism.
Experimental Design: The total number of 36 rats was divided into six groups, each groupcontaining six. The Group 1 is normal rats treated as control. Group 2 was DEN (200 mg/kg bw)induced rats and phenobarbital 0.05% was used as a promoter. Group 3 was treated withHinokitiol alone 50 mcg/kg/bw. Group 4 was treated as DEN with low dose of Hinokitiol 25mcg/kg/bw and phenobarbital 0.05%. Group 5 was treated as DEN with medium dose ofHinokitiol 50 mcg/kg/bw and phenobarbital 0.05%. Group 6 was treated as DEN with high doseof Hinokitiol 75 mcg/kg/bw and phenobarbital 0.05%. Rats from all groups were assessed thehaematological profile, biochemical parameters, mineral contents and also the genes like COX 2,IL6, TNF alpha, iNOS and P53.
Results: The Haematological Profile, Biochemical Parameters, Mineral Contents of the rats backto normalcy after treating with hinokitiol. Hinokitiol exerts its anticancer effects via inhibition ofinflammatory markers such as P53, TNF alpha, IL6, iNOS and COX2 which were involved ininflammation-associated carcinogenesis.
Conclusion: Restoration of Haematological Profile, Biochemical Parameters and MineralContents to normal levels following Hinokitiol treatment indicates that it will improve parameterlevels and inhibit Hepatocarcinogenesis. Inhibition of inflammatory markers also evidenced thatthe Hinokitiol can be used as new therapeutic implications for hepatocarcinogenesis.
Keywords: Hinokitiol, Hepatocarcinogenesis, Inflammatory mechanism, Genes, Biochemical parameters