World Summit on COVID-19 (Part V)
  • Follow

Accepted Abstracts

SN-RAMP Assay: A Low-Cost Isothermal Colorimetric Assay for Rapid and Reliable Bed Side Diagnosis of COVID-19 from Non-Invasive Clinical Samples

Kauser Banu, Nisha S, Bhairab Mondal* and Raju H
Shankaranarayana Life Sciences LLP, India.

Citation: Banu K, Nisha S, Mondal B, Raju H (2022) SN-RAMP Assay: A Low-Cost Isothermal Colorimetric Assay for Rapid and Reliable Bed Side Diagnosis of COVID-19 from Non-Invasive Clinical Samples. SciTech Central COVID-19.

Received: June 02, 2022         Accepted: June 06, 2022         Published: June 06, 2022

Abstract

The COVID-19 is a newly emerged highly infectious coronavirus that has never been found in humans before. The World Health Organization has classified the COVID-19 outbreak as a Public Health Emergency of International Concern. In India, approximately 136 laboratories are working currently on COVID 19. In the present study we have developed a COVID-19 closed-tube SN-RAMP assay platform for use in the hospitals, clinics and at ports of entry and even at patient home because this would require minimally trained personnel without a need for sophisticated equipment. Using bioinformatics, we have identified highly conserved sequences in the COVID-19 and designed primers targeting the open reading frame 1ab ORF1ab gene of the COVID-19 RNA virus. The assay required minimal sample processing. The health care provider can collect nasal samples with a swab and can carry out RNA extraction. Further, the sample will be inserted into our designed tube for two-stage amplification at 37 °C and 63°C. The incubation can be carried out with simple instrumentation like a heat block device as no thermal cycling is needed. The test results were monitored colorimetrically with HNB dye that can change colour from pink in the absence of dsDNA to purple colour in the presence of abundant amplified dsDNA. This colour change will be visible to the naked eye and will not require any instrumentation. Moreover, the amplification process would be monitored in real-time with the fluorescent dye, enabling quantification if needed in a laboratory setup. Our method will be relatively more sensitive than PCR based assays, including RT-PCRs which are presently in use. All reagents would be readily stored in the tube in dry form, providing long, refrigeration-free shelf life.