Background: The ability of plant extract to improve injury in the liver has gained interest in recent times. This could be due to the side effects and expense of modern medicines that are used to manage hepatic diseases; hence beetroot juice as a potential hepatoprotective agent was evaluated.  Materials and Methods: Thirty Sprague–Dawley rats were assigned by weight into six groups average body weight (160.20 ± 2.54 g). Group I: rats plus distilled water (Normal control) 2 ml/kg; Group II: rats received olive oil (2 ml/kg); Group III: rats received carbon tetrachloride (CCl₄) suspended in olive oil (2 ml/kg) and 250 mg/kg of beetroot extract. Group IV: rats received CCl₄ suspended in olive oil (2 ml/kg) and 500 mg/kg of  beetroot extract. Group V: rats received olive oil (2 ml/kg) and 100 mg/kg of silymarin; Group VI: rats received (2 ml/kg) CCl₄ suspended in olive oil. Liver injury was induced by oral administration of CCl₄ using gastric gavage at 2 ml/kg every 48 h for 14 days, followed by treatment with beetroot extract and silymarin. Animals were euthanized by decapitation, blood and liver tissue harvested for biochemical and histopathological evaluations. Results: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels were significantly (P < 0.05) increased in the groups treated with extract and silymarin when compared to the animals administered only CCl₄, whereas malondialdehyde level was significantly (P < 0.05) increased in the group administered only CCl₄ when compared to other groups. Histopathologically, the animals treated with 500 mg/kg of extract showed a normal  hepatocyte and  mild portal congestion. Conclusion: Beetroot juice has potential hepatoprotective effects on the liver in a dose‑dependent manner.

Keywords: Beetroot juice: carbon tetrachloride: hepatoprotective: liver injury