International Conference on Biomedical and Cancer Research
  • Follow

Accepted Abstracts

Assessenent of Bioctives and Cytotoxic Potential of Ficus Asperifolia and Phyllantus Amarus of Aqueous and Ethanolic Leave Extract using Brine Shrimp (Artemia Salina) Model

Emmanuel T Friday1*,Chidozie V.O2
1 Kogi state university,  Nigeria
2 Salem University, Nigeria


Citation: Emmanuel TF (2019) Assessenent of Bioctives and Cytotoxic Potential of Ficus Asperifolia and Phyllantus Amarus of Aqueous and Ethanolic Leave Extract using Brine Shrimp (Artemia Salina) Model, SciTech Biomed-Cancer Sciences 2019. Tokyo: Japan

Received: July 02, 2019         Accepted: July 04, 2019         Published: July 04, 2019

Abstract

The pyhtochemical constituents, mineral compositions and cytotoxicity studies of Ficus asperifolia and Pyllanthus amarus were investigated. The aqueous and ethanolic leaf extracts were used for pytochemical studies The leaf powders were used for mineral determination while brine shrimps (artemia salina) model were used for the toxicity investigation. Phytochemical studies carried out showed the presence of alkaloids,saponis,tannins,cardiac glycosides,anthocynins, , terpenoids and triterpenes in aqueous extracts of F.asperiofolia(AqFa) andP.amarus(AqPa) while phenolics,alkaloids,flavonoids,saponin,coumarins,phlobatannins,glycosides,terpenoids,triterpens and fixed oil are present in ethanolic extracts of F.asperiofolia(EtFa) and P. amarus(EtPa). flavonoid,steroids and coumarins were not detected in aqeuous extract of both leaves. The findings further showed that the value of total phenolics compound ranged between 10 - 64mg/100g,alkaloids 10 - 50 mg/100g,flavonoids 44 - 48.50mg/100g,saponnins 27 - 61.80mg/kg, tannins 0.07-1.10g/100g in aqueous extract both leaves respectively. AqFa,AqPa,EtFa, and EtPa coumarins 27 .10 - 71.50µg/100g,Anthocynins 0.19 - 0.27,glycosides 3.1 - 8.80mg/100g steroids 0 - 51.60mg/100g,terpenoids 60 - 88.14µg/100g and ,triterpenes 41 - 78.70 µg/100g in aqueous and ethanolic extracts of both plant leaf. The results show that both plant leaf have substantial amount of all selected major minerals (K,Na,Mg,Ca) and little amount of trace element (Cr,Cu,Fe,Pb).The results obtained showed that percentage lethality rate in ethanolic extract was more in aqueous extract in both extracts with increased in hours of exposure to brine shrimps. The extracts( leaf(AqFa,AqPa,EtFa,and EtPa with LC 50 86.66,44.51,37.33 and 144.89µg/ml respectively) were relatively safe when compared with reference potassium dichromate (LC 50 30.30 µg/ml). This studies therefore, has confirmed and justified the use of the preparations of Ficus asperifolia and Phyllanthus Amarus amongst the people especially those in the rural communities where the practice has becomes prevalence owing to easy accessibility to the plant and the relatively low cost of the preparations.