International Conference on Oncology & Hematology
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Accepted Abstracts

Monitoring endogenous antioxidant enzyme status in cancer is essential? A research report

Raju Asirvatham*
St. Joseph’s College of Pharmacy, India

Citation: Asirvatham R (2019) Monitoring endogenous antioxidant enzyme status in cancer is essential? A research report. SciTech Oncology 2019. Dubai: UAE

Received: April 04, 2019         Accepted: April 05, 2019         Published: April 08, 2019

Abstract

Mutations in DNA and lipids peroxidation are the major causes found in cancer development. In this malondialdehyde is an end products of lipid peroxidation act as a co-carcinogenic agent due to its high cytotoxicity and inhibitory action on protective enzymes promote tumor invasion. The chronic inflammation is always associated with malignant process that leads to generation of abundant source of free radicals resulting in further genetic instability. Therefore, oxidative stress is closely related to the occurrence and development of various types of cancer. Various tissue endogenous antioxidants enzymes appear to ease oxidative stress by scavenging reactive oxygen species before they cause damage to the various biological molecules or prevent oxidative damage from spreading e.g. by interrupting the radical chain reaction of lipid peroxidation. Inadequate level of these endogenous antioxidants enzymes also affects the mortality of cancer survivals. Hence, antioxidants and lipid peroxidation status in patients must be measured during treatment. Medicinal plants with high phenolics, carotenoids and flavonoids phytoconstituents have been shown to possess functional properties such as antimicrobial, antimutagenic, and free radical scavenging activity. They are potential anticancer agent acts serve as natural antioxidant, prevent the free radical formation, protect the endogenous antioxidant enzymes and increase the mortality of cancer survivals. Under the natural category one of the flavonoid rich plant is Drosera. Drosera is an insectivorous plant and consists of approximately 170 species. In India, Drosera indica L., Drosera burmannii Vahl and Drosera peltata J.E.Sm. have been reported at different locations. The present study was aimed to evaluate the antioxidant role of Drosera indica L. in Dalton Ascites lymphoma (DAL) bearing Swiss albino mice. Swiss albino mice were divided in to seven group of ten each. All the animals in groups 2- 6 were injected with DAL cells (2x106cells per mouse/i.p.), except first group. Group 1 served as the normal control. Group 2 served as the DAL control. Group 1 and 2 received normal diet and water. Group 3 served as the positive control and was treated with injection 5- Fluorouracil (5FU) at 20mg/kg body weight, intraperitonealy. Group 4 served as the treatment control, which was treated with ethanol extract of D. indica L (EEDI) at 250mg/kg body weight orally. Group 5 served as the treatment control, which was treated with EEDI at a dose of 500mg/kg body weight orally. Group 6 served as treatment control which was treated with aqueous extract of D. indica L (AEDI) at 250mg/kg of body weight, through orally. Group 7 served as treatment control which was treated with AEDI at 500mg/kg of body weight orally. In this study, drug treatment was given after the 24h of inoculation, once daily for 14days. After the treatment period, blood was collected from the animals and subsequently the animals were sacrificed for isolation of liver, brain, kidney and lungs for the observation of antioxidant status level. The parameters analyzed were catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), peroxidase (P), total protein (TP) and protein carbonyl content (PCC). Treatment with EEDI and AEDI significantly reduced the levels of MDA and increased the levels of GSH, SOD, CAT, P and TP in cancer induced animal and are similar as that of normal mice. The results suggest that the ethanol and aqueous extract of D. indica L. possess significant antioxidant effects in DAL bearing mice. This study concluded that plant components which contribute in combating the oxidative stress and free radical induced damage and protection of biological system from the oxidative stress induced disease such as cancer. Improved antioxidant status helps to minimize oxidative damage, and thus can delay or prevent pathological changes.  It may be possible that the natural antioxidants strengthen the endogenous antioxidant defense from ROS destroy and restore the optimal balance by neutralizing the reactive species.