International Conference on Oncology & Hematology
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Accepted Abstracts

Metformin attenuates oxidative stress and liver damage after bile-duct ligation in rats

Heibatollah Sadeghi1, Fatemeh Jahanbazi1, Hossein Sadeghi1, Navid Omidifar2, Behnam Alipoor1, Esmaeel Panahi Kokhdan1, Seyed Mehdi Mousavipoor1, Seyed Hossein Mousavi-Fard3, Amir Hossein Doustimotlagh1

 

1 Yasuj University of Medical Sciences, Yasuj, Iran
2 Shiraz University of Medical Sciences, Shiraz, Iran
3 Texas A&M University, College Station, Texas, USA

Citation: Sadeghi H, Jahanbazi F, Hossein S, Omidifar N, Doustimotlagh AH et al (2019) Metformin attenuates oxidative stress and liver damage after bile-duct ligation in rats.. SciTech Oncology 2019. Dubai:UAE

Received: April 22, 2019         Accepted: April 23, 2019         Published: April 24, 2019

Abstract

The aim of the current study was to investigate the antioxidative effect of metformin (Met) on bile duct ligation (BDL)- induced hepatic disorder and histological damage in rats. The rats were divided into 4 groups including SC (sham control), BDL alone (BDL surgery), Met1 (BDL surgery and administration of 250 mg/kg of metformin) and Met2 (BDL surgery and administration of 500 mg/kg of metformin). After BDL, the animals treated with Met by gavage for 10 days. Hematoxylin and eosin staining, biochemical analysis and oxidative stress markers were assayed to determinate histological alterations, liver functions and oxidant/antioxidant status. Hepatotoxicity was verified by remarkable increase in plasma levels of aminotransferases and alkaline phosphatase activity and liver histology 10 days after the BDL surgery. Our finding showed that treatment with Met markedly reduced plasma ALP and alleviated liver injury indexes (P≤ .05). Furthermore, BDL caused a considerable increase in the protein carbonyl and malondialdehyde content (P≤ .05). However, metformin reduces oxidative stress by constraining the protein oxidation and lipid peroxidation, and increases antioxidant reserve by increasing the ferric reducing ability of plasma and reduced glutathione levels.  Metformin exerts antioxidative effects in the liver fibrosis and may represent a hepatoprotective effect when given to rats with BDL-induced hepatic injury.
Key words: Oxidative stress; Cholestasis; Fibrosis; Metformin; Antioxidant