35th World Summit on COVID-19 (Part VI)
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Accepted Abstracts

Lung-Brain Axis: Metabolomics and Pathological Changes in Lungs and Brain of Respiratory Syncytial Virus Infected Mice

Mao Yu1,2, Ousman Bajinka1,2,3*, Zhongxiang Tang1,2, Xiangjie Qiu1, 2and Yurong Tan1, 2

1Department of Medical Microbiology, Central South University, Changsha, Hunan Provinces, China.

2China-Africa Research Center of Infectious Diseases, School of Basic and Medical Sciences, Central South University, Changsha 410078, Hunan, China.
3School of Medicine and Allied Health Sciences, University of the Gambia, Gambia.

Yu M, Bajinka O, Tang Z, Qiu X, Tan Y (2022) Lung-Brain Axis: Metabolomics and Pathological Changes in Lungs and Brain of Respiratory Syncytial Virus Infected Mice. SciTech Central COVID-19.

Received: September 21, 2022         Accepted: September 23, 2022         Published: September 23, 2022


Background: The lung-brain-axis is an emerging area of study that got its basis from the gut-brain-axis biological pathway.
Objective: Using Respiratory Synctial Virus (RSV) as the model of respiratory viral pathogen, this study aims to establish some biological pathways.
Material and Methods: After establishing the mice model,the samples were sent to Wuhan GeneCreate Biological Engineering Co., Ltd for metabolomics analysis. In addition, Hematoxylin-eosin staining, indirect immunofluorescence (IFA) and RT-qPCR were also carried out to establish the pathological changes.
Results: RSV infection promoted epithelial shedding and infiltration of inflammatory cells. Also, RSV immunofluorescense and titres were significantly increased. Moreover, IL-1, IL-6 and TNF-αwere also significantly increased after RSV infection and the cell structure of hippocampal CA1 area was loose and disordered. Inflammatory cytokines IL-6 and IL-1βexpression in the brain also increased however, TNF-αexpression showed no differences among the control and RSV group.We observed an increased expression of IBA-1 and decreased NeuN neuronal. In addition, RSV mRNA expression levels were also increased and15 metabolites were found upregulated in the RSV group including nerve-injuring metabolite glutaric acid, hydroxyglutaric acid and Spermine.  Finally, ɑ-Estradiol increased significantly while normorphinedecreased significantly at day 7 of infection among the RSV group.
Conclusion: This study established a mouse model for the metabolomics model, and utilised hematoxylin-eosin staining, IFA and RT-qPCR to establish the pathological changes. It reported epithelial shedding and infiltration of inflammatory cells post RSV infection, along with RSV immunofluorescence and increased titres.
Keywords: Lung brain axis, Metagenomics, Metabolomics, Rsv infection, Neurons
Abbreviations: RSV: Respiratory Synctial Virus; IFA: Indirect Immuno Fluorescence