The study also deals with the proximate chemical investigation of the roots with various extracts such as petroleum ether to water. The anthelmintic property of the methanolic extract of plant carried out using Pheretima posthuma as a test worm. Major observation in present bioassay was determination of time of paralysis and time of death. The proximate chemical investigation results showed that the presence of carbohydrates, protein, glycosides- flavonoid, phenols, tannins, terpenoids and keto steroids. The glycosides, tannins and terpenoids may be responsible for anthelmintic activity. The presence study includes anthelmintic activity of Orthosiphon thymiflorus whole plant first time. 

Key words: Orthosiphon thymiflorus, Flavonoids, Terpenoids, Anthelmintic