Silver nanoparticles was considered a powerful antimicrobial agents recently especially, after increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens which necessary required to find a novel path to eradicate that’s challenge. The present study aims to biosynthesize silver nanoparticles (SNPs) using a cell-free extract of Enterobacter cloacae and chemosynthesis by NaBH₄ (Sodium borohydride) as reduction agent and evaluate their antibacterial activity on biofilm forming Pseudomonas oryzihabitance and in combination with imipenem antibiotics. Antimicrobial effect of silver nanoparticles in both types and in combination with imipenem were evaluated by agar well diffusion. The results revealed that’s a good response of biofilm Pseudomonas oryzihabitance toward silver nanoparticles in both types and in combination with imipenem, the antimicrobial effect was increased and enhanced. From this study, the biological and chemical silver nanoparticles were considered a novel and decisive solution against biofilm and multidrug-resistant bacteria with a preference of biological silver nanoparticles as well as in combination with imipenem.