In the present work, Nano-mechanical properties of intermetallic layer formed on 316L austenitic stainless steel have been investigated. Al-Si alloys with varying percentage of silicon i.e. 8%, 10%, 12%, and 14%, using hot-dip method. Nano-mechanical properties of the Intermetallic formed have been evaluated using Nano-indentation technique at room temp era tu re and results were compared. On the basis of our observations and obtained results it is concluded that with increasing silicon percentage the thickness of the intermetallic layer increases. Howev er, the Na no-mechanical results showed that this layer is brittle in nature. Therefore, it can be inferred th at for bio-implant application the thickness of the lay er needs to be at lower level as possible.