Seminal plasma(SP) proteins are rich with many proteins of different genital tract origin so the fields of proteomics were  promise for the development of novel male infertility biomarkers. Seminal plasma proteins Testis Expressed Protein 101   (TEX101) and Extracellular Matrix Protein 1(ECM1) assay  are already available or under final development for clinical use,so the aim of study, evaluation of TEX101& ECM1 seminal plasma(SP) proteins for assessment the  predictive of sperm retrieval rate (SRR) in testicular sperm retrieval and diagnosis obstructive from non- obstructive azoospermia .

A case control  study was  included 65 infertile azoospermic men  were  subjected to  clinical examination , seminal fluid analysis , hormonal investigation and SP proteins TEX101& ECM1 assessment by Enzyme Linked Immuno-sorbent Assay (ELISA) as well as they were subjected to the conventional testicular sperm extraction(TESE) technique, mincing with searching for sperm.

The result of study included  mean age of 65 men were recorded  33.37 ± 6.99 years which were  divided into 10 (15.38%) obstructive type (OA) and 55(84.62) non-obstructive (NOA) type .The SRR account  36 out of 65 patients (55.4 %) were divided into OA (100 %) & NOA (47.3 %), and the difference was significant (P = 0.014) . The TEX101 & ECM1 were  a significantly (P< 0.001 & P = 0.007 respectively), higher in NOA than OA.The receiver operating characteristic curve, or ROC curve show that the SP TEX101 cutoff  values above 0.9 ng/ml is candidate to sperm retrieval technique.The ECM1 protein , the cutoff values(>943.11pg/ml for differentiation of  NOA versus OA.

Keywords: Proteomics,TEX101,ECM1,Sperm retrieval technique, Reproductive hormones , Testicular histopathology.