World Congress on Immunology & Microbiology
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Accepted Abstracts

Diversity and symbiotic effectiveness of rhizobium isolates collected from different Faba bean (Vicia faba) growing areas of north and south Gondar, Ethiopia

Zewdu Teshome Awlachew*, Samuel Sahile Woldemariam
University of Gondar, Ethiopia

Citation: Awlachew ZT, Woldemariam SS (2019) Diversity and symbiotic effectiveness of rhizobium isolates collected from different Faba bean (Vicia faba) growing areas of north and south Gondar, Ethiopia. SciTech Immuno-Microbiology 2019. Dubai: UAE

Received: January 30, 2019         Accepted: February 04, 2019         Published: February 04, 2019

Abstract

Fifty seven Rhizobium isolates were retrieved based on infection method from different faba bean growing areas of North and South Gondar, Ethiopia. In sand culture, only 35.5% of isolates showed nodulation. Analyses of variance indicated that inoculation of isolates improved tested traits significantly (p< 0.05) at all measured investigated parameters such as shoot length, shoot dry weight, and plant total nitrogen as 11, 28 and 31.3%, respectively, over nitrogen treated plants, 2, 10 and 29.4% respectively over standard  Rhizobium  and  55,  82.3  and  85.7%  respectively over negative  control treatments.  Shoot  dry  weight  was  found  to  be strongly  positively  correlated  with symbiotic effectiveness (r = 0.994, P <0.01). Based on symbiotic effectiveness, 80% of the tested isolates were found to be highly effective, 13% effective and only one isolate less effective. Therefore, t here is a need for detail study of effective isolates tested under field conditions and molecular characterization for better classification.

Key words: Authentication, Legumes, Nitrogen fixation, Rhizobium, total nitrogen.